Isocitrate Lyase Activity in Thiobacillus versutus Grown Anaerobically on Acetate and Nitrate
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چکیده
منابع مشابه
Nitrate reductase from anaerobically grown Rhizobium japonicum.
The activity of nitrate reductase in Rhizobium japonicum is controlled by oxygen tension, and not by nitrate. The enzyme from R. japonicum grown anaerobically in the presence of nitrate resembles that from bacteroids in having a molecular weight of about 69000 daltons; the enzyme from aerobically grown cells ahs a molecular weight of about 170000 daltons. Both types of enzyme have similar Km va...
متن کاملInteraction of acetate, glucose and growth conditions on glyconeogenesis and isocitrate lyase activity in Tetrahymena.
-1. Incubation of shaken cultures for 3 hr with acetate had little effect on the level of isocitrate lyase or the capacity for glyconeogenesis. 2. Maintenance of cultures under static conditions for the same period led to an eight to tenfold increase in the enzyme and to a greatly enhanced ability to convert acetate to glycogen. 3. Glucose prevented most of the increase in enzyme and led to a c...
متن کاملColonial heterogeneity of Thiobacillus versutus.
In acetate-limited chemostat cultures started with single-colony cultures of Thiobacillus versutus, a mutant appeared after approximately 85 volume changes. The inhomogeneity of the culture was detected by the development of two different types of colonies on agar plates. When a pure culture of the mutant was grown in a chemostat, parent colonies appeared after almost the same period of time. E...
متن کاملGenetic regulation of isocitrate lyase activity in Aspergillus nidulans.
The formation of the glyoxylate cycle enzymes isocitrate lyase and malate synthase is strongly regulated in Aspergillus nidulans and the enzymes are induced at high levels in mycelium grown on acetate and found at low levels in mycelium grown on hexose. A search was made for constitutive mutants forming the enzymes when grown on hexose by the use of a pyruvate carboxylaseless (pycA) strain. Thi...
متن کاملGenetic control of isocitrate lyase activity in Escherichia coli.
Isocitrate lyase (EC 4.1.3.1) plays a necessary role in the growth of Escherichia coli on acetate as sole carbon source (1). Pyruvate and phosphoenolpyruvate both inhibit the activity and repress the synthesis of this enzyme (5). Mutants in which the synthesis of isocitrate lyase is no longer subject to control by these C3-compounds, and which thus form isocitrate lyase constitutively, have bee...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1986
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-132-11-3179